As I mentioned in my last update, last Thursday I joined the lab group at the Triangle Chromatography Discussion Group in Raleigh, NC. This was a great experience! For my college friends…I had to get up at 5am to get there on time. The day was filled with various presentations mainly focusing on natural chemistry like cannabinoids, natural fungi, wine chemistry, and more. Between the presentations, I got to mingle with the numerous analytical chemistry vendors like Waters and Agilent in the vendor expo (got some free promo stuff). The day was long, however, filled with some interesting topics.
Back in the lab, I began my UV/Visible absorbance studies with the SQBA dye and three simple sugars (fructose, ribose, and arabinose). We were collecting absorbance data to determine the excitation wavelength by testing the following solutions: dye only, each sugar only, and a complex of dye and sugar. After some data collection and analysis in Excel…I had my excitation wavelength of 618 nm! Now I can use that to collect Fluorescence emission data in dye only and dye/sugar complex solutions. The goal in Fluorescence studies is to determine the emission wavelength and emission parameters to use in method development for capillary electrophoresis (CE) separation. My emission wavelength turned out to be 656 nm!
All in all, after a few days I completed the UV/Vis and Fluorescence data giving me many data charts to place in my lab notebook (It is very important to keep excellent lab notebook records!). Check back with me soon as I begin my CE method development.